高压脉冲电场提取猪皮胶原蛋白

为了研究高压脉冲电场(PEF)对提取猪皮胶原蛋白的影响,在料液比1:10、枯草杆菌中性蛋白酶用量400U/g、酶解温度40℃、pH值6.5的条件下,分别改变电场强度、脉冲频率和酶解时间,用考马斯亮兰法测定胶原蛋白的浓度并计算其溶出率.结果表明,最适电场强度为25kV· cm-1、脉冲频率为40Hz、脉冲时间1s、酶解时...     

4种促性腺激素释放激素抗体的制备、鉴定和纯化

用3-马来酰亚胺基苯甲酸琥珀酰亚胺酯(MBS)法将人工合成的4种不同来源的促性腺激素释放激(GnRH)(即章鱼GnRH、海鞘GnRH-Ⅰ、七鳃鳗GnRH-Ⅰ和七鳃鳗GnRH-Ⅲ)分别与匙孔戚血蓝蛋白(KLH)偶联后作为抗原,免疫新西兰大白兔获得GnRH抗体.用间接ELISA方法检测抗血清效价,并用Western blot鉴定其特异性.制备抗原肽亲和纯化柱,纯化多克隆抗体.结果显示,4种多克隆抗体效价高达1∶500 000(体积比),并能和相应的GnRH多肽特异性结合,纯化后的抗体在SDS-PAGE显示为单一条带.通过上述方法,获得4种高效价、高纯度的GnRH抗体,为低等动物GnRH的研究提供了理论依据.     

使用伪氨基酸组成和BP神经网络预测类弹性蛋白多肽的相变温度

根据获得的16条ELP序列及相变温度的数据,利用伪氨基酸组成方法提取其序列特征值.将伪氨基酸组成中的相关系数部分作为类弹性蛋白的特征向量,从类弹性蛋白序列出发,利用最小中位方差回归,找出与其序列相关系数的最佳阶数.运用均匀设计法,分别对支持向量机与BP神经网络参数进行优化.结果表明:BP神经网络获得的预测模型最佳,相变...     

Skin collagen fiber-based radar absorbing materials

By using skin collagen fiber (CF) as raw material,Schiff base structure containing CF (Sa-CF) was synthesized through CF-salicylaldehyde reaction.Then a novel radar absorbing material (Fe-Sa-CF) was prepared by chelating reaction between Sa-CF and Fe 3+.The coaxial transmission and reflection method was used to analyze the complex permittivity and complex magnetic permeability of these CF-based materials,and the radar cross section (RCS) method was used to investigate their radar absorbing properties in the frequency range of 1.0-18.0 GHz.Experimental results indicated that the conductivity of CF increased from initial 1.08×10-11 to 2.86×10-6 S/cm after being transferred into Fe-Sa-CF,and its dielectric loss tangent (tanδ) in the frequency range of 1.0-17.0 GHz also increased.These facts suggest that the Fe-Sa-CF is electric-loss type radar absorbing material.In the frequency range of 3.0-18.0 GHz,Sa-CF (1.0 mm in thickness) exhibited somewhat radar absorbing property with maximum radar reflection loss (RL) of-4.73 dB.As for Fe-Sa-CF,the absorbing bandwidth was broadened,and the absorbing intensity significantly increased in the frequency range of 1.0-18.0 GHz where a maximum radar RL of-9.23 dB was observed.In addition,the radar absorbing intensity of Fe-Sa-CF can be further improved by increasing membrane thickness.When the thickness reached to 2.0 mm,the RL values of Fe-Sa-CF were-15.0-18.0 dB in the frequency range of 7.0-18.0 GHz.Consequently,a kind of novel radar absorbing material can be prepared by chemical modification of collagen fiber,which is characterized by thin thickness,low density,broad absorption bandwidth and high absorption intensity.     

戊二醛交联纳米羟基磷灰石/胶原复合材料作用机理研究

为了改善纳米羟基磷灰石／胶原复合材料界面结合性能，采用戊二醛对复合材料进行交联处理，并对交联前后复合材料的溶胀度，结合能进行了分析，研究戊二醛交联对羟基磷灰石／胶原复合材料溶胀度的影响，探讨了戊二醛交联羟基磷灰石／胶原复合材料的可能机理．结果表明，复合材料的溶胀度随戊二醛的浓度增加而降低，当戊二醛的浓度大于0．25％时，复合材料的溶胀度没有明显的变化．戊二醛交联促使羟基磷灰石与胶原之间形成更多键，导致O1s，Ca2p，N1s，P2p和C1s的化学状态和结合能变化，增加了胶原纤维与羟基磷灰石之间的界面结合强度。     

两栖动物皮肤抗菌肽的研究进展

两栖动物的皮肤抗菌肽是抵御外界微生物侵袭的有效屏障之一.此文综述了两栖动物皮肤抗菌肽的最新研究进展,重点介绍了天然抗菌肽的分离、纯化、基因克隆与表达的过程,并展望了其在农业和临床上的应用前景.     

不同干燥方法对鲍鱼营养成分的影响

为研究不同干燥方式对鲍鱼中营养成分的影响,采用自然晾晒、热风干燥和微波真空干燥的方式,研究干制鲍鱼中多糖、总糖、胶原蛋白以及氨基酸含量的变化情况。结果表明,自然晾晒的鲍鱼中多糖和总糖含量显著高于热风干燥及微波真空干燥,而胶原蛋白及氨基酸含量则显著低于其余2种干燥方法。其中胶原蛋白含量以微波真空干燥的方式最佳,但同热风干燥间相差不大;氨基酸含量以热风干燥方式最佳。综合整体实验结果,热风干燥及微波真空干燥优于自然晾晒,且有利于工业化生产加工。     

草鱼多肽功能性质及营养价值

研究草鱼多肽的溶解性、起泡性、乳化性、吸湿保湿性和稳定性等功能性质,以及草鱼多肽的氨基酸组成,并评价其营养价值。结果表明草鱼多肽具有良好的功能特性。草鱼多肽粉18种氨基酸总质量分数为90.94%,必需氨基酸占总氨基酸质量分数的41.33%;草鱼多肽的生物价、必需氨基酸指数、营养指数和氨基酸比值系数分别为71.51,76.34,74.81和77.19。草鱼多肽的氨基酸组成平衡、合理,是一种优质的蛋白质资源。     

Effects of isaxonine phosphate and analogs on fibroblast metabolism in culture

Summary Human skin fibroblasts in confluent cultures were incubated for 24 h in the presence of isaxonine phosphate (Nerfactor) and several related factors. The incorporation of¹⁴C-proline into secreted proteins and the release of collagen into the medium were inhibited. When the cells incubated for an additional period of 24 h after thorough washing, protein and collagen syntheses were found to be identical to those of controls, demonstrating that the inhibition of protein synthesis was independent of any toxic effect. When cells were incubated in the presence of both isaxonine and colchicine, the secretion of collagen was more inhibited than by colchicine alone, and proteins accumulated in the cells.